(PDF) A Coprological Diagnostic Comparison …
Zinc Sulfate flotation method showed the higher efficiency in diagnosing intestinal parasites (36.64%), while among sedimentation methods which were used in the present study Pomegranate molasses ...
A COMPARISON OF THE ZINC SULFATE AND THE …
The zinc sulfate flotation tgohnic as developed by thoroughly mixed with 10 ml. of 7.5% fomalin and Faust et all and Tobie et al has been widely used allowed to stand 10-30 minutes. ... stool concentration method that acceptably fulfilled Formalin is decanted following centrifugation and the dual role of recovering both eggs and cysts. Re ...
11.3.4 Microscopic Examination of Fecal Specimens: Concentration …
11.3.4 Microscopic Examination of Fecal Specimens: Concentration by Zinc Sulfate Flotation. Author: Wilson W Chan; Wilson W Chan. Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Canada. Google Scholar. More articles by this author . Last updated: 9 May 2023.
Comparison Formalin-Ethyl Ether Sedimentation, …
zinc sulfate procedure. Onthe other hand, the zinc sulfate procedure failed to detect Taenia eggsin onespecimenand Trichuriseggs in one oftwo specimens. In addition, the morphology ofGiardia cysts wasbetter preserved with the sedimentation procedures than with the flota-tion procedure. In addition to the concentration procedures
Understanding the effect of sulphate in mining-process …
The purpose of this kinetic-bulk flotation was to obtain a rougher Cu concentrate with depression of Zn and Fe minerals with the addition of zinc sulphate at a high pH of 11.8. Fig. 10 depicts the Cu grade versus Cu recovery curves (after 3, 8 and 15 min of flotation) at the three sulphate concentrations. Cu recoveries are high reaching 96% in ...
Parasitology Exam 1 Questions Flashcards
After concentration of stool, only an iodine wet preparation is examined because. saline does not provide enough contrast for detection of parasites. One major advantage of the formalin-ethyl acetate concentration procedure over the zinc …
CDC
Each flotation solution have variable efficacy for individual parasite species and references should be consulted before choosing the ideal solution for detecting specific …
Feasibility of flotation concentration of fungal spores as a …
Flotation concentration of spores in feces followed by microscopic evaluation may overcome this problem and has been used to confirm mushroom exposure in humans and animals. ... We found that zinc sulfate flotation of partially digested mushroom samples yielded spore numbers adequate for microscopic visualization in 97.5% of trials where a ...
A COMPARISON OF THE ZINC SULFATE AND THE …
The zinc sulfate flotation tgohnic as developed by thoroughly mixed with 10 ml. of 7.5% fomalin and Faust et all and Tobie et al has been widely used allowed to stand 10-30 minutes. Fixation …
Comparative evaluation of a modified zinc sulfate flotation …
Abstract. Comparative evaluation of a modified zinc sulfate flotation technique. M S Bartlett, K Harper, N Smith, P Verbanac and J W Smith ABSTRACT A modified zinc sulfate flotation technique using Formalinized fecal specimens (F-ZnSO4) was compared to the Formalin-ether (FE) concentration method for the laboratory diagnosis of intestinal parasites.
zinc sulfate flotation concentration
a method using saturated zinc sulfate to separate parasitic elements from fecal debris through differences in specific gravity; most parasitic cysts, oocysts, spores, eggs, and larvae can be found in the surface film after centrifugation.
Concordance between the zinc sulphate flotation …
The faecal concentration methods based on sedimentation of parasites are useful for recovering heavy parasite eggs due to the concentration of the organisms in the sediment, whereas the centrifugal-flotation in zinc sulphate (9) is used …
Stool Examination:- Part 2
- Formalin-ethyl-acetate concentration method.
- Zinc-floatation method.
- Sheather sugar floatation method.
Flotation Technique for Fecal Parasite …
By exploiting the density of the parasites, particularly eggs, it allows the parasites to float to the top of a dense solution (final specific gravity of about 1.20) and can then be skimmed from the top of the tube. The most commonly used reagent is …
Comparison of Common Fecal Flotation Techniques for …
1.20), magnesium sulfate (MgSO 4; SG 1.20), and zinc sulfate (ZnSO 4; SG 1.20). These solu-tions are effective, easy to make or commercially available, and relatively inexpensive. Flotation procedures vary from the simple to the complex. The simplest procedure involves mixing a small amount of feces with flotation
Zinc Sulfate
Zinc sulfate (ZnSO 4 ·xH 2 O) is produced by reacting ZnO with sulfuric acid (H 2 SO 4). It is the most commonly used Zn fertilizer and it is available in the monohydrate and heptahydrate forms. ... Zinc supplementation in children improves mean serum zinc concentration, improves height, reduces diarrhea morbidity, and leads to a small ...
Concentration Techniques for stool examination .pptx
The high sugar concentration increases the specific gravity of the solution, causing the parasites to float. Zinc sulphate floatation Zinc sulphate or (Sheather's sugar) use solutions which have higher specific gravity than the organisms to be floated so that the organisms rise to the top and the debris sinks to the bottom.
CONCENTRATIONS TECHNIQUES IN …
10. 3. Pour off the clear supernatant, add a small quantity of zinc sulfate solution (specific gravity 1.18- 1.2) and resuspend the sediment well. 4. Add zinc sulfate solution to a little below the brim and centrifuge at 2,500 …
General Parasitology and Helminthology: Floatation technique
Concentration methods. Sedimentation technique. Floatation technique. Interpretation of microscopic examination of faeca... Examination of urine. Examination of blood for detection of microfilaria. Knott's concentration method. Haematocrit method. Cellophane tape preparation. Examination of nasal washings. Topic 55. Topic 56. Topic 57. Topic ...
Concordance between the zinc sulphate flotation and
The faecal concentration methods based on sedimentation of parasites are useful for recovering heavy parasite eggs due to the concentration of the organisms in the sediment, whereas the centrifugal-flotation in zinc sulphate 9 is used preferentially to recover protozoa cysts because of the low density of parasitic forms as compared to the salt ...
Comparison of Routine and Stool Concentration …
parasitic detection after concentration in our study. Protozoan cysts were observed by direct microscopy method and two concentration methods (zinc sulphate floatation and …
JCDR
Various concentration techniques like simple slat floatation, Zinc sulphate centrifugal floatation, formol-ether concentration and modified formol-ether concentration are employed for the …
Concentration Techniques : Introduction, Principle, Types, Test Procedure
Concentration by flotation Ova and cyst of parasites having a density lower than that of suspending medium are floated at the top of the medium. Saturated sodium chloride (Brine solution) and 33% zinc sulfate (ZNSO4) are suspending media.
International Journal of Current Microbiology and …
Zinc sulfate flotation concentration technique was found to be most sensitive method in this study, then came Formal-Ethyl acetate at rate of (43%) and direct wet mount (22.3%) as shown in Table 2. Interestingly males were more infected with intestinal parasites (57.28%) than females (50%).as shown in Table 3. Children under
Concentration Techniques : Introduction, Principle, …
Saturated sodium chloride (Brine solution) and 33% zinc sulfate (ZNSO4) are suspending media. Concentration by sedimentation can be achieved by the following methods- Formalin- Ether or Formalin- Ethyl acetate method is the …
Flotation Technique for Fecal Parasite Concentration Methods
3. Resuspend and thoroughly mix the sediment in 12ml of zinc sulphate solution (specific gravity, 1.18 to 1.20, as verified with a hydrometer). 4. Centrifuge for one minute at 2500 rpm. Place tube in a rack in a vertical position and slowly add enough zinc sulphate with a dropper pipette to fill the tube so that an inverted meniscus forms. 5.
Concordance between the zinc sulphate flotation and …
Additionally, the fecal concentration technique was performed with a zinc sulfate concentration of 33.0% for the concentration of eggs (2 g of feces were homogenized in 10 mL of water, filtered ...
Comparison of Formalin-Ethyl Ether Sedimentation, Formalin …
The concentration procedures that constitute the main techniques applied in routine research and in parasitological kits are a) spontaneous sedimentation; b) centrifugation-sedimentation with formalin-ethyl acetate; and c) flotation with zinc sulfate solution.
Concordance between the zinc sulphate flotation and …
The faecal concentration methods based on sedimentation of parasites are useful for recovering heavy parasite eggs due to the concentration of the organisms in the sediment, whereas the centrifugal-flotation in zinc sulphate 9 is used preferentially to recover protozoa cysts because of the low density of parasitic forms as compared to the salt ...
Feasibility of flotation concentration of fungal spores as a …
In phase 1, quantitative spore recovery rates were determined following magnesium sulfate, modified Sheather's sugar solution, and zinc sulfate flotation (n=16 fungal species). In phase 2, mushrooms (n=40 fungal species) were macerated and digested for up to 2 hours in a salivary and gastric juice simulant.
Comparative evaluation of a modified zinc sulfate flotation
A modified zinc sulfate flotation technique using Formalinized fecal specimens (F-ZnSO4) was compared to the Formalin-ether (FE) concentration method for the laboratory diagnosis of …