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Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one ...
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Stretchable light-emitting materials are the key components for realizing skin-like displays and optical biostimulation. All the stretchable emitters reported to date, to the best of our knowledge ...
The invention provides a high-efficiency convenient charger, which is characterized in that: the outer surfaces of the charger (1) and the mobile phone shell (2) are provided with at least one conductive binding post (3), or electrode plate (4), or cambered surface electrode (18), or mobile phone finger ring (32), and the binding post (3), or electrode plate (4), or cambered surface …
Electroporation, which has high transformation efficiency requires special and expensive equipment [9, 10], so the establishment of a convenient and rapid chemical method of transformation for all E. coli bacterial strains and large DNA fragments is necessary and important to the development of modern molecular biology.
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Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one ...
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Adsorption is an economic, efficient, and simple technique because of its advantages of fast removal, high efficiency, and low environmental effect [12], [13]. ... Herein, a one-step, high-speed shear method (HSSM) was proposed to prepare an efficient, convenient, nontoxic, and green aqueous nitrate adsorbent with high adsorption capacity. The ...
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A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex targeting in plants Xingliang Ma 1,4, Qunyu Zhang 1,2,4, Qinlong Zhu 2,4,Wei Liu 1,2,4, Yan Chen 5, Rong
A robust CRISPR/Cas9 vector system for multiplex genome editing in monocot and dicot plants was developed. Multiple sgRNA expression cassettes can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning. This system can uniformly, efficiently, and simultaneously produce multiple heritable mutations in rice and Arabidopsis by targeting …
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A high efficiency furnace is a type of heating system that operates at a much higher efficiency level than standard models. Generally, these furnaces achieve an Annual Fuel Utilization Efficiency (AFUE) rating of 90% or higher.
Additionally, by changing the thickness of the orange unit, we modulated the exciton recombination zone in the structure of the WLEDs to realize high efficiency. As a result, based on a 4 nm orange unit, we demonstrated high-efficiency two-color perovskite/organic hybrid WLEDs with a maximum EQE of 13.54 % and luminance of 28,503 cd/m 2.
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A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol. Plant. 2015; 8:1274-1284. Full Text. Full Text (PDF) Scopus (1619) PubMed. Google Scholar. 7.
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DOI: 10.1016/j.molp.2015.04.007 Corpus ID: 25184623; A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. @article{Ma2015ARC, title={A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.}, author={Xingliang Ma and Qunyu …