Tissue Pulverizer Liquid Nitrogen

Spectrum™ Bessman Tissue Pulverizers

After chilling the mortar and pestle in liquid nitrogen, the included 28 cm lead hammer is used on the mortar to completely pulverize the inserted sample. ... 3 sizes available: 10-50mg, 25-250mg and 100-1000mg of tissue; ... Large Pulverizer includes one 90 mL culture jar; Specifications. Specifications. Capacity (Metric) 10 to 50 mg: Material ...

Preparing for a metabolomics experiment using tissues

Polar metabolites extraction from tissues . Decide the best way to homogenize your tissues: (I) pulverize the tissue in liquid nitrogen(LN2) using a mortar and pestle or alternatives (see below), or (II) use a bead beating method. Practice this beforehandso that you know it will work for your tissue types and what challenges there might be! 1.

59014N 316 Stainless Steel BioPulverizer with Hammer, 1 …

In a typical procedure, up to 10 g of animal or plant tissue is hard-frozen in liquid nitrogen and placed in the pre-chilled BioPulverizer. The piston delivers a blow to the brittle tissue reducing it to powder. Freeze fracturing with the Bio-Pulverizer is also useful for extracting labile tissue metabolites.

Tissue sample preparation for proteomic analysis

A freeze-fracturing tissue pulverizer is a typical example of a stainless-steel grinder (e.g., BioPulverizer, Bio Spec Products Inc., OK, USA). It rapidly fragments previously frozen tissue samples into powder in the range of 0.1–10 mg. 31 Liquid nitrogen is used to make the tissue hard and brittle. The frozen tissue is then inserted into the ...

BioPulverizer Instructions

Slowly add liquid nitrogen to the stainless steel parts until nitrogen gas no longer flashes off and some liquid nitrogen lingers on the stainless steel. Add appropriate sized pre-frozen (-80 °C or lower) tissue to the well of the mortar. Fresh tissue sticks to the cold BioPulverizer on contact and cannot be inserted into the well.

BioSpec Products 59012N Tissue Pulverizer, 10 to 100 mg tissue …

This tissue pulverizer reduces samples frozen in liquid nitrogen to a coarse powder. Use this powder as a preliminary step for cell disruption (homogenization) and metabolite, protein and nucleic acid extraction. Available in 2 sizes for tissues samples of 10 mg to 1 g.

BioSpec Products 59012N Tissue Pulverizer, 10 to …

This tissue pulverizer reduces samples frozen in liquid nitrogen to a coarse powder. Use this powder as a preliminary step for cell disruption (homogenization) and metabolite, protein and nucleic acid extraction. Available …

SOP: Nuclei preparation from liquid nitrogen snap …

SOP: Nuclei preparation from liquid nitrogen snap-frozen tissue using a Bessman tissue pulverizer Date modified: 04/01/2013 Modified by: T. Canfield (UW) Ordering Information The following protocol describes the isolation of nuclei from liquid nitrogen snap-frozen tissue (mouse or human) using a Bessman tissue pulverizer.

Metabolite extraction from tissues using LN2 pulverization

3. Cut the tissue in smaller pieces (see above), transfer the pieces to a cold microcentrifuge tube, and quickly snap-freeze the sample in liquid nitrogen (LN2) for 5 min. 4. Store the tissues in a -80 ˚C freezer until ready for further processing. ----- 5. Homogenize the tissues by pulverization in liquid nitrogen (WEAR PROPER PPE!)

'Cool Tools' for Cryopulverizing Tissue and Microbial …

The base and piston are pre-cooled to liquid nitrogen (liq N2) temperatures ard pre-frozen animal or plant tissue is placed inside the chilled hole. The chilled piston is placed on top of the hard frozen tissue sample, and with a few sharp blows with a hand-held hammer, the piston fragments normally fibrous or rubbery tissue into particles the ...

BioPulverizer

The Bio-Pulverizer reduces tissue hard-frozen in liquid nitrogen to a fine powder. Called freeze-fracturing, this method is especially useful for tough, fibrous tissues such as skin, cartilage, cornea, etc. The Bio-Pulverizer consists of a hole …

Repligen Bessman Tissue Pulverizer

Description The stainless steel Bessman Tissue Pulverizer consists of a 2 component mortar with handles and a pestle, specifically designed for pulverizing 10 - 1000 mg of tissue. After chilling the Pulverizer in liquid nitrogen, the included 28 cm lead hammer is used on the mortar to completely homogenize the inserted sample. The sample can then

Cellcrusher video, tissue pulverizer workflow for cell …

Tissue pulverizer video. This step-by-step video demonstrates the Cellcrusher workflow. The curved surfaces of the device allow for effective sample recovery and cleaning at liquid nitrogen temperatures. The Cellcrusher is can be used to pulverize a wide sample size range (10 mg to 5 g), which is possible because of these curved surfaces. ...

Tissue pulverizer for breaking tough tissue samples in liquid nitrogen

Particularly tough tissue types (such as seeds or skin) are not completely disrupted by pulverization with the Cellcrusher. For such samples, secondary grinding with the drill-bit accessory is often necessary This tool can be used with any variable speed drill or electric screwdriver that can hold a 9 mm bit.

BioSpec Products 59013N Tissue Pulverizer, 0.1 to 1 g tissue …

This tissue pulverizer reduces samples frozen in liquid nitrogen to a coarse powder. Use this powder as a preliminary step for cell disruption (homogenization) and metabolite, protein and nucleic acid extraction. Available in 2 sizes for tissues samples of 10 mg to 1 g.

Cell and Tissue Preparation for NAD Metabolomics …

Bessman Pulverizer (Spectrum Labs, Catalog 988-14500 (Small) and 988-14512 (Large)) b. Hammer that comes with the Bessman Pulverizer c. Curved Tweezers ... Place the frozen samples into a Styrofoam container that contains a large amount of liquid nitrogen. 2. Pulverize tissue using a Bessman Pulverizer. The following URL will take you to a youtube

Protocol for lysate preparation from mouse tissues for

Homogenize the mouse tissue in liquid nitrogen using a pulverizer. Add 4% SDS lysis buffer [ 2% SDS in 50mM TEABC, Protease inhibitor cocktail ] to the homogenized powder and heat at 90°C for 5min.

Tissue pulverizer for small samples, RNA and …

With six chambers, each only 8 mm wide, this tissue pulverizer offers unparalleled efficiency when crushing and recovering tiny samples at liquid nitrogen temperatures. To avoid potential confusion, the top edge of the device is …

Protocol for total protein extraction from various mouse or rat tissues

Tissues: Homogenize the tissue in liquid nitrogen using a pulverizer. Add 4% SDS lysis buffer [ 2% SDS in 50mM TEABC, Protease inhibitor cocktail ] to the homogenized powder and heat at 90°C for ...

Liquid Nitrogen – Medical Applications | NitrogenX

🌡️ Cryosurgery: This minimally invasive technique uses liquid nitrogen to remove abnormal tissues, making it invaluable in the treatment of certain cancers and benign conditions. ️ Diagnostic Medicine: Liquid Nitrogen enables the cooling and preservation of various medical specimens, allowing for more accurate diagnostic tests and research.

Bessman Tissue Pulverizer, Spectrum® Laboratories

The stainless steel Bessman Tissue Pulverizer consists of a 2 component mortar with handles and a pestle, specifically designed for pulverizing 10 - 250 mg of tissue. 2 sizes 10 - 50 mg and 25 - 250 mg available; Includes 10 test tubes; After chilling the mortar and pestle in liquid nitrogen, the included 28 cm lead hammer is used on the mortar ...

010458 Tissue processing with the CP01 cryoPREP® …

The CP01 cryoPREP Manual Dry Pulverizer can be used to process tissue samples within a mass range of 15 mg to 1 g. TT05M - A single-use, sample pulverizing tube designed with a threaded hub for rapid transfer of contents into a milliTUBE. The TT05M may be directly immersed in liquid nitrogen or stored at -80C. (PN: 520139)

Homogenizing tissue using cryoPREP

This Procedure & checklist describes tissue homogenization using the Covaris cryoPREP Automated Dry Pulverizer. Extraction data is provided from various human tissue samples. ... the tissue is incubated in liquid nitrogen. • Tissue is pulverized with 10 impacts on maximum force interspersed with 10 second incubations in liquid

Simple apparatus for pulverization and rapid quantitative transfer …

The pulverizer consists of a mortar, mortar base plate and pestle containing a milled section (Fig. 1). The base plate is made of stainless steel while the mortar and pestle are made of anti-corrodal aluminum or stainless steel. ... It is essential that the tissue be frozen solid with liquid nitrogen for good pulverization. Tissues frozen only ...

Scienceware® Liquid Nitrogen Cooled Mini Mortar

Pulverize tissue and extract DNA or RNA from tiny tissue samples within a microcentrifuge tube. Stainless steel mortar has a welded fixture to hold a 1.5ml microcentrifuge tube. The mortar is suspended above a stainless steel cavity which holds the liquid nitrogen and is nested in a heavily insulated blue polyethylene housing.

CELLCRUSHER CELLCRUSHER KIT WITH TISSUE PU …

This ergonomically designed tissue pulverizer from Cellcrusher is used with liquid nitrogen to process varied tissue samples. This product option includes the tissue pulverizer and its accessories, a mallet, handling pliers and recovery spoon. …

US4509695A

A tissue pulverizer for pulverizing specimens of living tissues for facilitating chemical testing and the like. The pulverizer has a base, a guide member for emptying into a receiving tube and an elongated pestle member. The parts are cooled with liquid nitrogen and the specimen inserted, covered with the cooled pestle and frozen. The pestle member is then struck with a hammer to …

FAQs on the Cellcrusher tissue pulverizer, cell types, …

The drill-bit accessory is used for two main tissue types; (a) Very tough, often dry samples that are not fully disrupted by impact pulverization. Samples that contain water (such as cartilage) are extremely brittle when frozen with liquid nitrogen and are quickly and effectively disrupted by impact. Dry samples, such as seeds or bark benefit ...

Tissue Pulverizer 40355